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Enzo Biochem t7 rna transcript labeling kit
T7 Rna Transcript Labeling Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7+rna+transcript+labeling+kit/t7+rna+transcript+labeling+kit/pmc02648959-216-77-82
Average 90 stars, based on 1 article reviews
t7 rna transcript labeling kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Synthesized:

Article Title: Cloning of cytochrome p450 genes from
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: A functional profile of gene expression in ARPE-19 cells
Article Snippet: .. First and second strand cDNA were synthesized from 15 μg of total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (GIBCO, Carlsbad, CA) and oligo-dT24-T7 (5'-GGC CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG AGG CGG-3') primer according to the manufacturer's instructions. cRNA was synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc. Farmingdale NY). .. The fragmented cRNA was hybridized to the olygonucleotide array, washed, stained with phycoerythrein conjugated streptavidin (Molecular Probes, Eugene OR), and scanned.

Article Title: SW-620 cells treated with topoisomerase I inhibitor SN-38: gene expression profiling
Article Snippet: First- and second-strand cDNA were synthesized from 5–15 μg of total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Gibco Life Technologies) and oligo-dT24-T7 (5'-GGC CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG AGG CGG-3') primer, according to the manufacturer's instructions. .. Next, cRNA was synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double-stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: Cloning of cytochrome P450 genes from nicotiana
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: The blood transcriptome of childhood malaria
Article Snippet: .. Total RNA was extracted from whole blood using the PAXgene Blood RNA System (Qiagen, Hilden, Germany), pooled in equal amounts to a total of 5 μg from four individuals and the first- and second-strand cDNA was synthesized using the SuperScript Double-Stranded cDNA Synthesis Kit (Life Technologies Inc., Rockville, MD, USA) and an oligo-dT24-T7 primer. cRNA was prepared and labeled with biotinylated UTP and CTP by in vitro transcription using a T7 promoter-coupled double-stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc., Farmingdale, NY, USA). ..

Article Title: Cloning of cytochrome p450 genes from
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: Genomic analysis of human lung fibroblasts exposed to vanadium pentoxide to identify candidate genes for occupational bronchitis
Article Snippet: .. Biotinylated cRNA was synthesized from an aliquot of the cDNA template using the T7 RNA Transcript Labeling Kit (ENZO Diagnostics, Farmingdale NY). .. The labeled cRNA was then fragmented, hybridized to Affymetrix Human Genome U133A 2.0 arrays (Affymetrix, Santa Clara, CA), and stained using phycoerythrein-conjugated streptavidin (Molecular Probes, Eugene, OR).

Labeling:

Article Title: Cloning of cytochrome p450 genes from
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: A functional profile of gene expression in ARPE-19 cells
Article Snippet: .. First and second strand cDNA were synthesized from 15 μg of total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (GIBCO, Carlsbad, CA) and oligo-dT24-T7 (5'-GGC CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG AGG CGG-3') primer according to the manufacturer's instructions. cRNA was synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc. Farmingdale NY). .. The fragmented cRNA was hybridized to the olygonucleotide array, washed, stained with phycoerythrein conjugated streptavidin (Molecular Probes, Eugene OR), and scanned.

Article Title: SW-620 cells treated with topoisomerase I inhibitor SN-38: gene expression profiling
Article Snippet: First- and second-strand cDNA were synthesized from 5–15 μg of total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Gibco Life Technologies) and oligo-dT24-T7 (5'-GGC CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG AGG CGG-3') primer, according to the manufacturer's instructions. .. Next, cRNA was synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double-stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: Cloning of cytochrome P450 genes from nicotiana
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: The blood transcriptome of childhood malaria
Article Snippet: .. Total RNA was extracted from whole blood using the PAXgene Blood RNA System (Qiagen, Hilden, Germany), pooled in equal amounts to a total of 5 μg from four individuals and the first- and second-strand cDNA was synthesized using the SuperScript Double-Stranded cDNA Synthesis Kit (Life Technologies Inc., Rockville, MD, USA) and an oligo-dT24-T7 primer. cRNA was prepared and labeled with biotinylated UTP and CTP by in vitro transcription using a T7 promoter-coupled double-stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc., Farmingdale, NY, USA). ..

Article Title: Cloning of cytochrome p450 genes from
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: Genomic analysis of human lung fibroblasts exposed to vanadium pentoxide to identify candidate genes for occupational bronchitis
Article Snippet: .. Biotinylated cRNA was synthesized from an aliquot of the cDNA template using the T7 RNA Transcript Labeling Kit (ENZO Diagnostics, Farmingdale NY). .. The labeled cRNA was then fragmented, hybridized to Affymetrix Human Genome U133A 2.0 arrays (Affymetrix, Santa Clara, CA), and stained using phycoerythrein-conjugated streptavidin (Molecular Probes, Eugene, OR).

In Vitro:

Article Title: Cloning of cytochrome p450 genes from
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: A functional profile of gene expression in ARPE-19 cells
Article Snippet: .. First and second strand cDNA were synthesized from 15 μg of total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (GIBCO, Carlsbad, CA) and oligo-dT24-T7 (5'-GGC CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG AGG CGG-3') primer according to the manufacturer's instructions. cRNA was synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc. Farmingdale NY). .. The fragmented cRNA was hybridized to the olygonucleotide array, washed, stained with phycoerythrein conjugated streptavidin (Molecular Probes, Eugene OR), and scanned.

Article Title: SW-620 cells treated with topoisomerase I inhibitor SN-38: gene expression profiling
Article Snippet: First- and second-strand cDNA were synthesized from 5–15 μg of total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Gibco Life Technologies) and oligo-dT24-T7 (5'-GGC CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG AGG CGG-3') primer, according to the manufacturer's instructions. .. Next, cRNA was synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double-stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: Cloning of cytochrome P450 genes from nicotiana
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

Article Title: The blood transcriptome of childhood malaria
Article Snippet: .. Total RNA was extracted from whole blood using the PAXgene Blood RNA System (Qiagen, Hilden, Germany), pooled in equal amounts to a total of 5 μg from four individuals and the first- and second-strand cDNA was synthesized using the SuperScript Double-Stranded cDNA Synthesis Kit (Life Technologies Inc., Rockville, MD, USA) and an oligo-dT24-T7 primer. cRNA was prepared and labeled with biotinylated UTP and CTP by in vitro transcription using a T7 promoter-coupled double-stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc., Farmingdale, NY, USA). ..

Article Title: Cloning of cytochrome p450 genes from
Article Snippet: .. The cRNA was concurrently synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc.). ..

cDNA Synthesis:

Article Title: A functional profile of gene expression in ARPE-19 cells
Article Snippet: .. First and second strand cDNA were synthesized from 15 μg of total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (GIBCO, Carlsbad, CA) and oligo-dT24-T7 (5'-GGC CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG AGG CGG-3') primer according to the manufacturer's instructions. cRNA was synthesized and labeled with biotinylated UTP and CTP by in vitro transcription using the T7 promoter coupled double stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc. Farmingdale NY). .. The fragmented cRNA was hybridized to the olygonucleotide array, washed, stained with phycoerythrein conjugated streptavidin (Molecular Probes, Eugene OR), and scanned.

Article Title: The blood transcriptome of childhood malaria
Article Snippet: .. Total RNA was extracted from whole blood using the PAXgene Blood RNA System (Qiagen, Hilden, Germany), pooled in equal amounts to a total of 5 μg from four individuals and the first- and second-strand cDNA was synthesized using the SuperScript Double-Stranded cDNA Synthesis Kit (Life Technologies Inc., Rockville, MD, USA) and an oligo-dT24-T7 primer. cRNA was prepared and labeled with biotinylated UTP and CTP by in vitro transcription using a T7 promoter-coupled double-stranded cDNA as template and the T7 RNA Transcript Labeling Kit (ENZO Diagnostics Inc., Farmingdale, NY, USA). ..

other:

Article Title: Alterations in cell growth and signaling in ErbB3 binding protein-1 (Ebp1) deficient mice
Article Snippet: First and second strand cDNA were synthesized from 5–15 μg of total RNA at Genome Explorations (Nashville, TN) using the SuperScript Double-Stranded cDNA Synthesis Kit (Gibco Life Technologies) and an oligo-dT 24 -T7 (5'-GGC CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG AGG CGG-3') primer according to the manufacturer's instructions.



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